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ZHU Ya-Nan, ZHANG Shu-Wen, MAO You-Dong. Cryo-electron microscopy revolutionizes molecular biophysics[J]. PHYSICS, 2017, 46(2): 76-83. DOI: 10.7693/wl20170202
Citation: ZHU Ya-Nan, ZHANG Shu-Wen, MAO You-Dong. Cryo-electron microscopy revolutionizes molecular biophysics[J]. PHYSICS, 2017, 46(2): 76-83. DOI: 10.7693/wl20170202
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Cryo-electron microscopy revolutionizes molecular biophysics

  • Center for Quantitative Biology,School of Physics,Peking University,Beijing 100871,China

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  • Received Date: October 05, 2016
  • Published Date: February 11, 2017
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    • Abstract
  • Cryo-electron microscopy (cryo-EM) has become a widely used technology in biophysics. In recent years, with the application of the direct electron detector device camera and continuous innovations in the single-particle reconstruction algorithms, cryo-EM has made tremendous progress. Macromolecular structures can now be determined with near atomic resolution (<4 Å) with this technique. As more and more proteins and complexes with important biomedical implications are being revealed with high-resolution, structural biology is undergoing a gradual revolution. Cryo-EM is attracting worldwide attention. In this article we describe its development and single-particle analysis techniques, taking the inflammasome complex and splicesome as examples to demonstrate the latest advances.
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