Cryo-electron microscopy revolutionizes molecular biophysics

ZHU Ya-Nan; ZHANG Shu-Wen; MAO You-Dong

doi:10.7693/wl20170202

PHYSICS > 2017 > 46(2): 76-83. > DOI: 10.7693/wl20170202

ZHU Ya-Nan, ZHANG Shu-Wen, MAO You-Dong. Cryo-electron microscopy revolutionizes molecular biophysics[J]. PHYSICS, 2017, 46(2): 76-83. DOI: 10.7693/wl20170202

Citation:

ZHU Ya-Nan, ZHANG Shu-Wen, MAO You-Dong. Cryo-electron microscopy revolutionizes molecular biophysics[J]. PHYSICS, 2017, 46(2): 76-83. DOI: 10.7693/wl20170202

Citation:

ZHU Ya-Nan, ZHANG Shu-Wen, MAO You-Dong. Cryo-electron microscopy revolutionizes molecular biophysics[J]. PHYSICS, 2017, 46(2): 76-83. DOI: 10.7693/wl20170202

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Cryo-electron microscopy revolutionizes molecular biophysics

Center for Quantitative Biology,School of Physics,Peking University,Beijing 100871,China

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Received Date: October 05, 2016
Published Date: February 11, 2017

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Abstract

Abstract

Cryo-electron microscopy (cryo-EM) has become a widely used technology in biophysics. In recent years, with the application of the direct electron detector device camera and continuous innovations in the single-particle reconstruction algorithms, cryo-EM has made tremendous progress. Macromolecular structures can now be determined with near atomic resolution (<4 Å) with this technique. As more and more proteins and complexes with important biomedical implications are being revealed with high-resolution, structural biology is undergoing a gradual revolution. Cryo-EM is attracting worldwide attention. In this article we describe its development and single-particle analysis techniques, taking the inflammasome complex and splicesome as examples to demonstrate the latest advances.
- cryo-EM,
- single-particle analysis,
- molecular biophysics,
- structural biology

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